The pcr amplification products were separated on 6 % vertical denaturing polyacrylamide gels . numerous and distinct bands could be detected by silver staining Pcr扩增产物经6变性聚丙烯酰胺凝胶垂直电泳分离后，银染能检测到多而清晰的条带。
The aflp ( amplified fragment length polymorphism ) technique is based on the selective pcr amplification of restriction fragments from genomic dna digested by restriction enzymes completely 扩增片段长度多态性技术( aflp )是基于选择性扩增完全酶切消化后的基因组dna片段。
Confirmation of transformant . integration of sag - ipt into chrysanthemum genome was confirmed in two thirds of the putative tansgenic plantlet be pcr amplification 通过pcr扩增表明，转基因植株中可以检测出有目的基因( ipt基因)的存在，目的基因已成功地插入菊花的基因组中。
Plant defensin afp ( 238bp ) was amplified with six long complementary primers after two rounds of pcr amplification and plant expression vector peafp was constructed . 3 通过合成六条长链引物，经两次pcr扩增，获得了238bp的植物防御素afp基因全长，并构建了植物表达载体peafp 。
Further we cloned full - length cdna of the ked like protein coding region via pcr amplification and confirmed its interaction with acam2 in yeast two - hybrid system 进一步通过rt . pcr克隆了ked样蛋白编码区全长cdna ，通过双杂交验证了全长ked样蛋白与acamz的相互作用，结果为阳性。
By means of pcr amplification to total dna extracted from four transgenic plants , it indicated that bet - a gene had been integrated into the haploid of the hybrid genome 最终得到四个转化株系，经pcr检测，全部产生特异性扩增谱带，证明bet - a基因已整合进小黑杨单倍体植株的基因组中。
With the improved method of ctab , the genomic dna extracted form plum and other species close to p . mume in genetic relationship could be directly used in pcr amplification , with good results obtained 选用改良ctab法提取的梅及其近缘种的基因组dna可直接用于pcr扩增分析，且效果良好。
We cloned full - length cdna of ca coding region via pcr amplification , but a negative result was obtained when the interaction of full - length ca and ga was tested in yeast two - hybrid system 通过rt - pcr克隆了ca编码区全长cdna ，但是在双杂交系统检测全长ca与g蛋白的相互作用时，结果为阴性。
E2 gene was cut with restriction endonuclease from the recombination plasmid t - e2 , and subcloned into the expression vector ppic9 ' s mcs , which was identified by enzyme digestion and pcr amplification 将重组质粒t - e2的e2基因酶切后，亚克隆到表达载体ppic9上，经酶切和pcr鉴定，命名为ppic9 - e2 。
It also was a cosmopolitan species in the northern hemisphere . the phylogenetics relationships between the isolates of a . gallica from europe , north america and china were studied with issr - pcr amplification 并对这5个蜜环菌生物种的地理分布、生长规律、寄主范围、培养特性等生物学习性进行了研究讨论。
